The 4th Hansenula polymorpha Worldwide Network Conference, Haren (The Netherlands), 3â5 September 2006
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Business Process Management, 4th International Conference, BPM 2006, Vienna, Austria, September 5-7, 2006, Proceedings
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Using an optimized transformation protocol we have studied the possible interactions between transforming plasmid DNA and the Hansenula polymorpha genome. Plasmids consisting only of a pBR322 replicon, an antibiotic resistance marker for Escherichia coli and the Saccharomyces cerevisiae LEU2 gene were shown to replicate autonomously in the yeast at an approximate copy number of 6 (copies per ge...
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The metabolism of methanol was monitored in whole cells of the methylotrophic yeast Hansenula polymorpha by using [13C]methanol and n.m.r. in vivo. The main products observed under normal conditions were trehalose and glycerol, whereas cells that were starved before exposure to [13C]methanol also accumulated glutamate, glutamine and alanine; formate was also more prominent in spectra from starv...
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ژورنال
عنوان ژورنال: FEMS Yeast Research
سال: 2007
ISSN: 1567-1356,1567-1364
DOI: 10.1111/j.1567-1364.2006.00200.x